Journal: Biochemical and biophysical research communications
Article Title: Choline kinase alpha regulates autophagy-associated exosome release to promote glioma cell progression.
doi: 10.1016/j.bbrc.2024.151269
Figure Lengend Snippet: Fig. 3. CHKA mediates exosomes secretion in glioma cells. A-C Western blot (WB) analysis of exosome marker proteins CD63 and TSG101 in exosomes isolated from the supernatant of glioma cells in shCHKA and shNC group along with the negative control protein Calnexin. D Exosome protein concentration in the shCHKA and shNC groups of glioma cells using the BCA assay. E Electron microscopy was performed on shCHKA and shNC cells in glioma cells (Scale bars = 1 μm). F The number of multivesicular bodies (MVBs) per glioma cell profile. G The number of intraluminal vesicles (ILVs) per MVB profile in glioma cells. *P < 0.05, **P < 0.01, ***P < 0.001. shCHKA: CHKA knockdown group, shNC: con trol group.
Article Snippet: Antibodies used in the Western blotting process included: rabbit antihuman CHKA polyclonal antibody (abcam, USA), rabbit anti-human GAPDH polyclonal antibody (Affinity, China), rabbit anti-human CD63 polyclonal antibody (Proteintech, China), rabbit anti-human TSG101 polyclonal antibody(Proteintech, China), rabbit anti-human calnexin polyclonal antibody(Proteintech, China), rabbit anti-human LC3B polyclonal antibody(Sigma, USA), and rabbit anti-human P62/SQSTM1 polyclonal antibody(Sigma, USA).
Techniques: Western Blot, Marker, Isolation, Negative Control, Protein Concentration, BIA-KA, Electron Microscopy, Knockdown